Shenzhen Biorunstar Biotechnology Co., Ltd

 

Biotin labeling

Biotin can be attached to the peptide N-terminus or the C-terminus (via lysine or cysteine). Due to the very strong non‐covalent interaction between biotin and avidin / streptavidin, it is useful to have this small molecule as a tag to immobilise the peptide, or to pull‐down the peptide from a mixture. Note that a spacer, such as a mini‐PEG or Ahx (aminohexanoic acid), between biotin and the N‐terminus give a better performance in biotechnological applications generally.

 

Tagged Peptides

Peptide tags are widely used in protein expression and purification, and also be used to protein detection through Western blot, ELISA, ChIP, immunocytochemistry, immunohistochemistry, and fluorescence measurement.

 

Tag Peptide

 

Cell-penetrating peptides

Cell-penetrating peptides has been developed as an in vitro and in vivo delivery vectors to be used to facilitate localization to specific cellular compartments (membranes, cytoplasm).

 

Peptide Amidation and Peptide Acetylation

If the peptide originates from an internal protein sequence, In the protein, the peptide fragment's ends would be amides (CONH2), so amidating the C-terminus and acetylating the N-terminus mimics this natural state. These modifications eliminate the peptide's charge and aid in protecting it from enzymatic degradation.

 

list of some CPPs:

 

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